Last updated Sept 2, 2014 www.healthnz.co.nz/milk.pdf

A1 beta-casein a threat to dairy industry

Teasing out the beta-casein evidence

by In the Fairfax NZ Sunday Star Times

Professor Keith Woodford: I advocated that the mainstream dairy industry should convert New Zealand herds away from the production of A1 beta-casein. To not do so creates unnecessary long term risk to the industry. However, the mainstream industry remains locked into a defensive position.

In this article I will therefore briefly review some of the major strands of health evidence. I cannot cover it all – it took me a whole book to do so back in 2007. Since then, there has been a lot more evidence forthcoming.

In assessing the evidence, it is helpful to recognise that A1 beta-casein is the consequence of a historical mutation. Goats, sheep camels, buffalo, Asian cattle and humans produce beta-casein that is totally of the A2 type. It is only cows of European ancestry which produce A1 beta-casein.

In modern dairy herds, the proportion of A1 beta-casein varies by country, by breed and by herd. In New Zealand, there has been a slow drift towards A2. About 40 percent of New Zealand cows now produce beta-casein that is all A2, and most of the rest produce a 1:1 ratio of A1 and A2 beta-casein. A few animals produce only A1 beta-casein.

None of this would matter if it were not that A1 beta-casein on digestion releases a peptide (a protein fragment) called beta -casomorphin-7 (BCM7), whereas this does not occur with A2 beta-casein. Even the European Food Safety Report in 2009 conceded that this is correct. There is also no doubt that this peptide has opioid characteristics. It is a well-established scientific fact.

However, what has remained controversial until recently has been whether or not the BCM7 can pass through into the blood. Russian researchers have now shown quite clearly that it does pass into the blood of babies fed infant formula. They have also shown that a proportion of these babies are unable to metabolise the BCM7 efficiently between feeds and these particular babies have delayed psycho-motor (brain-to-muscle) development.

Russian workers have also found BCM7 in the urine of all children on normal milk diets. Polish researchers have even found that mothers who are themselves drinking cow milk can pass bovine BCM7 to their babies in breast milk.

The original evidence implicating A1 beta-casein came from Professor Bob Elliott from Auckland University. He noted that Samoan children brought up in Samoa had a minimal level of Type 1 diabetes whereas children of Samoan ethnicity in New Zealand are vulnerable. He looked for differences in lifestyle, and identified exposure to cow milk as a possibility. Subsequently working with Dr Murray Laugesen, he showed that across the developed world more than 80% of the between-country variations in Type 1 diabetes could be explained by per capita intake of A1 beta-casein. Corran McLachlan showed similar correlations between intake of A1 beta-casein and heart disease. The correlations are statistically very strong and no alternative explanation for these between-country differences has stood the test of time.

A human clinical trial from Curtin University in Australia, recently published in the European Journal of Clinical Nutrition, found that there were statistically significant differences in digestive symptoms between milks containing A1 and A2 beta-casein. This has drawn attention back to some of the animal trials for explanations. For example, a New Zealand trial with rats, undertaken by AgResearch and co-funded by the New Zealand Government and The a2 Milk Company, and published earlier this year, found increased levels of an inflammation marker MPO in the colon on the A1 diet. I am a co-author on both the Curtin and AgResearch papers.

A similar study with mice, published last year in the European Journal of Nutrition, found comparable inflammation results. That study also found strong immunological responses to the A1, with greatly increased levels of antibody production.

The New Zealand ‘AgRats’ study also found, as expected, that the opioid effects of BCM7 from A1 beta-casein slowed down the passage of food through the rat intestines. Intriguingly, the A1 beta-casein also significantly increased the release of an enzyme called DPP4. The reason this is so intriguing is that the modern gliptin drugs, now widely used to control Type 2 diabetes, act by inhibiting this enzyme, whereas with A1 beta-casein the level increased.

There is a lot more research of relevance, including arterial plaque in rabbits and increased antibodies to oxidised LDL in humans. I now have several hundred published studies of relevance in my database. There is also a stream of additional studies in the pipeline about which I am very excited. There is no chance this issue will go away.

Next week, in the last of this series on A1 beta-casein, I will explain how to eliminate production of A1 beta-casein through breeding. I will also look again at the industry politics of A1 beta-casein and why the industry is so defensive.

BCM7 in the milk we drink

Woodford, professor of farm management at Lincoln University, has reviewed a hundred scientific papers on the peptide betacasomorphin BCM7 in A1 milk, raising concerns about possible health effects. Research in 2003 by Laugesen and Elliott (see below) is featured, but recommendations by Professors Beaglehole, Jackson and Swinburn in 2003 for more research have yet to be implemented. The issue is not about whether people should drink milk but about whether people should be able to buy A1-free milk. A2 milk sales have increased in the North Island, but South Islanders cannot yet buy A2 milk at a reasonable price.

Devil in the Milk Illness, health and politics A1 and A2 milk. Professor Keith Woodford, Craig Potton Publishing ISBN 978-1-877333-70-5 – Oct. 07)

Data on BCM7 in New Zealand dairy products

Neither Fonterra nor A2 Corporation has published data on the BCM7 content of their milk products. The NZ Food Safety Authority repeats that milk is safe, but offers no test results to show that infant formula, for example, is free of BCM7.

Woodford’s book offers more than enough evidence to apply the precautionary principle, and he urges dairy farmers, at no extra cost, to use pure A2 semen from now on. For whatever one’s position on the science, once dairy farmers decide to inseminate their cows with pure A2 semen rather than with A1, the A1 content of New Zealand milk will decline to near zero (Guernsey Island levels) within 10 years. If Fonterra offered a slight premium at the farm gate for A2 milk, this goal would be reached much sooner.

Milk – is it safe?

The NZ Food Safety Authority’s re-iteration that milk is safe, is a generalization. Great care is taken to pasteurize it and keep it safe from communicable disease. With respect to non-communicable disease, milk, like most food additives and flavours, is Generally Regarded As Safe (GRAS). It is not, however, completely safe. For example, milk including A2 milk, can cause serious milk allergy.1

1. Smith WB, Thompson D, Kummerow M et al. Letter to MJA 2004 181 (10) 574.)

Research to reduce heart disease and diabetes

Laugesen and Elliott’s 2003 research paper confirms a high degree of correlation between A1 beta casein and heart disease and diabetes, at population level. This has raised the possibility that the type of casein in the fresh milk supply could be a risk factor. But proof of this concept is elusive. As Beaglehole and Jackson said in the accompanying NZMJ editorial, further research is recommended.

A1 but not A2 milk breaks down to form the peptide casomorphin-7. Much more needs to be known as to the final fate in the body of this peptide, known to be bioactive.

Health New Zealand’s research paper: www.nzma.org.nz/journal/116-1168/

NZ MJ editorial www.nzma.org.nz/journal/116-1168/

Fonterra’s comment: www.nzma.org.nz/journal/116-1169/

The authors’ reply: www.nzma.org.nz/journal/116-1170/

Heart disease and diabetes type 1 are commoner in North Europe, and one possible explanation may lie in the genetics of the cow and type of milk consumed. A1 milk differs very slightly from A2 milk in the composition of one of its main proteins, beta casein. A1 is a genetic variant of A2 milk.

A1 milk was commoner from black and white (Holstein-Friesian herds) or red and white herds, as found in Northern Europe, and A2 more in brown herds as in Southern Europe and the Channel Island breeds. These associations with skin colour have become blurred in recent decades by widespread artificial insemination from Holstein bulls. The proportion of A1 milk in the town supply still varies considerably across countries and somewhat over the years.

 

Further thoughts on A1 and A2 milk

Laugesen and Elliott found that while differences in A1 milk consumption can explain differences in heart disease and diabetes type 1 between countries, they do not explain why diabetes type 1 is increasing in almost all countries. Casomorphin 7 in A1 milk if glycated can be absorbed orally and have adverse immune effects. Recent data support the hypothesis that non-enzymatic pathways (glycation and oxidation) are involved in the pathogenesis of tissue damage in diabetes mellitus. Infant formula is high in advanced glycation end products (AGEs), which can cause diabetes in mice. A diet low in AGEs is protective in mice.

See Elliott RB. Diabetes – a man-made disease. Med Hypotheses. 2006;67(2):388-91. Epub 2006 Mar 10. http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=pubmed&cmd=Retrieve&dopt=AbstractPlus&list_uids=16530335&query_hl=1&itool=pubmed_docsum

Health New Zealand Ltd is interested in sponsors for further research on this topic.

A1 and A2 milk – what is the difference?

Health New Zealand on its own initiative, carried out research on the relation of A1 milk to heart disease, and type 1 diabetes, to prove or disprove the existence of correlations noted by Professor Bob Elliott and the late Dr Corran McLaughlin, later founder of A2 Corporation www.A2corporation.com which promotes A2 milk. If this correlation was true, it had importance for investors, for New Zealand, for public health and disease prevention. If not, then the sooner it was disproved the better. Fonterra’s Research Institute put its library at our disposal. Preliminary work showed the presence of strong correlations. The work was then completed with the assistance of A2 Corporation. The country-level correlations are not proof of concept, for which individual-level data were needed. This we acknowledge in our paper.

Publication of the Laugesen and Elliott paper in January 2003 resulted in several papers by Fonterra staff (Hill, Crawford) and nutritionists Mann (Otago) and Trusswell (Sydney), all critical of the A1/A2 concept. Cardiovascular epidemiologists, Beaglehole and Jackson, who wrote the editorial accompanying the paper, and cardiovascular nutritionist Swinburn who reviewed research to date on the issue, took the view that although correlations have their pitfalls, this correlation was of potentially great importance and deserving of further (commercially-funded) research to prove or disprove it.

When the paper below was published in 2003, A2 milk was not available in New Zealand, and even today it is only available in certain supermarkets. To a limited extent then, this research has secured greater consumer choice. As most of the top bulls are now pure A2, (whether by accident or design), the A2 content of the town milk supply will gradually increase.


J NZ Med J 24 January 2003; vol 116, no. 1168. Full text at www.nzma.org.nz/journal under Archived

Ischaemic heart disease, Type 1 diabetes, and cow milk A1 β-casein

Murray Laugesen and Robert Elliott

Abstract

Aim

To test the correlation of per capita A1 β-casein (A1/capita) and milk protein with: 1) ischaemic heart disease (IHD) mortality; 2) Type 1 (insulin-dependent) diabetes mellitus (DM-1) incidence.

Methods

A1/capita was estimated as the product of per capita cow milk and cream supply and its A1 β-casein content (A1/ β) (calculated from herd tests and breed distribution, or from tests of commercial milk), then tested for correlation with: 1) IHD five years later in 1980, 1985, 1990 and 1995, in 20 countries which spent at least US $1000 (purchasing power parities) per capita in 1995 on healthcare; 2) DM-1 at age 0–14 years in 1990–4 (51 were surveyed by WHO DiaMond Project; 19 had A1 data). For comparison, we also correlated 77 food, and 110 nutritive supply FAO (Food and Agriculture Organization)-based measures, against IHD and DM-1.

Results

For IHD, cow milk proteins (A1/capita, r = 0.76 , p <0.001; A1/capita including cheese, r = 0.66; milk protein r = 0.60, p = 0.005) had stronger positive correlations with IHD five years later, than fat supply variables, such as the atherogenic index (r = 0.50), and myristic, the 14-carbon saturated fat (r = 0.48, p <0.05). The Hegsted scores for estimating serum cholesterol (r = 0.42); saturated fat (r = 0.37); and total dairy fat (r = 0.31) were not significant for IHD in 1995. Across the 20 countries, a 1% change in A1/capita in 1990 was associated with a 0.57% change in IHD in 1995.

A1/capita correlations were stronger for male than female mortality. On multiple regression of A1/capita and other food supply variables in 1990, only A1/capita was significantly correlated with IHD in 1995.

DM-1 was correlated with supply of: A1/capita in milk and cream (r = 0.92, p <0.00001); milk and cream protein excluding cheese (r = 0.68, p <0.0001); and with A1/β in milk and cream (r = 0.47, p <0.05). Correlations were not significant for A2, B or C variants of milk β-casein. DM-1 incidence at 0–4, 5–9 and 10–14 years was equally correlated (r = 0.80, 0.81, 0.81 respectively) with milk protein supply. A 1% change in A1/capita was associated with a 1.3% change in DM-1 in the same direction.

Conclusions

Cow A1 β-casein per capita supply in milk and cream (A1/capita) was significantly and positively correlated with IHD in 20 affluent countries five years later over a 20-year period – providing an alternative hypothesis to explain the high IHD mortality rates in northern compared to southern Europe.

For DM-1, this study confirms Elliott’s 1999 correlation on 10 countries for A1/capita,1 but not for B β-casein/capita. Surveys of A1 β-casein consumption in two- year-old Nordic children, and some casein animal feeding experiments, confirm the A1/capita and milk protein/capita correlations. They raise the possibility that intensive dairy cattle breeding may have emphasised a genetic variant in milk with adverse effects in humans. Further animal research and clinical trials would be needed to compare disease risks of A1-free versus ‘ordinary’ milk.

 

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